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Related Concept Videos

The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

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Related Experiment Video

Updated: Jun 5, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

GSK3 regulates mitotic chromosomal alignment through CRMP4.

Stephan Ong Tone1, Bama Dayanandan, Alyson E Fournier

  • 1Department of Neurology and Neurosurgery, Montreal Neurological Institute, Montreal, Quebec, Canada.

Plos One
|December 24, 2010
PubMed
Summary

Collapsin Response Mediator Protein 4 (CRMP4) is a key substrate of Glycogen Synthase Kinase 3 (GSK3) during cell division. CRMP4 regulates chromosomal alignment and mitotic progression by affecting spindle microtubules.

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Related Experiment Videos

Last Updated: Jun 5, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Live Cell Imaging of Chromosome Segregation During Mitosis
06:39

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Glycogen Synthase Kinase 3 (GSK3) is implicated in cell division processes like chromosomal alignment and mitotic progression.
  • The specific substrates mediating GSK3's role in mitosis remain largely unidentified.
  • Collapsin Response Mediator Protein 4 (CRMP4), a known GSK3 substrate, regulates cytoskeletal dynamics.

Purpose of the Study:

  • To investigate the role of CRMP4 during mitosis.
  • To identify CRMP4 as a physiological substrate of GSK3 in mitotic regulation.

Main Methods:

  • Studied CRMP4 phosphorylation during mitosis.
  • Examined CRMP4 localization to spindle microtubules.
  • Assessed the impact of CRMP4 loss on chromosomal alignment and mitotic progression.
  • Investigated the dependence of CRMP4's function on GSK3 phosphorylation.

Main Results:

  • CRMP4 phosphorylation is regulated by GSK3 during mitosis.
  • CRMP4 localizes to spindle microtubules and is essential for proper chromosomal alignment and mitotic progression.
  • The function of CRMP4 in chromosomal alignment is dependent on GSK3 phosphorylation.
  • CRMP4 influences spindle microtubule regulation.

Conclusions:

  • CRMP4 is identified as a critical physiological substrate of GSK3 in the context of mitosis.
  • CRMP4 plays a key role in regulating chromosomal alignment and mitotic progression.
  • CRMP4 exerts its function through modulation of spindle microtubule dynamics.