Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

G-Protein Gated Ion Channels01:21

G-Protein Gated Ion Channels

GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory organs,...
Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Antihypertensive Drugs: Action of Calcium Channel Blockers01:18

Antihypertensive Drugs: Action of Calcium Channel Blockers

Calcium ions are essential to contract smooth muscle cells in blood vessels. They enter these cells through voltage-dependent calcium channels, specifically L-type calcium channels in the cell membrane. These L-type calcium channels are integral to the excitation-contraction coupling process in smooth muscle. When a stimulus is received by smooth muscle cells, their membrane depolarizes. This alteration in membrane potential instigates the opening of L-type calcium channels. As a result,...
Resting Membrane Potential01:24

Resting Membrane Potential

The relative difference in electrical charge, or voltage, between the inside and the outside of a cell membrane, is called the membrane potential. It is generated by differences in permeability of the membrane to various ions and the concentrations of these ions across the membrane.
The Inside of a Neuron is More Negative
The membrane potential of a cell can be measured by inserting a microelectrode into a cell and comparing the charge to a reference electrode in the extracellular fluid. The...
Regulation of Sodium and Potassium01:26

Regulation of Sodium and Potassium

The regulation of sodium and potassium ion concentrations in the human body is a complex process governed primarily by hormones such as aldosterone, antidiuretic hormone (ADH), and atrial natriuretic peptide (ANP).
Sodium Regulation
Sodium ions make up approximately 90% of extracellular cations, with a normal blood plasma concentration of 136–148 mEq/L. A decrease in blood volume and pressure triggers the release of renin from granular cells in the juxtaglomerular complex (JGC), primarily in...
The Resting Membrane Potential01:21

The Resting Membrane Potential

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Safety and preliminary efficacy of autologous bone marrow-derived mesenchymal stem cell transplantation in hereditary cerebellar ataxia: phase I/IIa clinical trial.

Stem cell research & therapy·2026
Same author

Aberrant TMPRSS6-Protease Regulation of Disease Mutant HCN4-KCNE1 Channel Complex Depends on the KCNE1-G38S Polymorphism.

Archiv der Pharmazie·2026
Same author

Deciphering the Cellular Effects of Strontium Chloride and Potassium Carbonate on Induced Pluripotent Stem Cells and Their Derivative Cardiomyocytes.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Regulation of Vascular Tone of Preglomerular Renal Vasculature by Caldesmon.

Journal of the American Heart Association·2026
Same author

A Nanoformulation of Ubiquinol and Selenium Promotes Proliferation of Human Induced Pluripotent Stem Cells.

Antioxidants (Basel, Switzerland)·2025
Same author

Vascular diameter determines sensitivity to soluble guanylate cyclase activation in human mesenteric and renal arteries.

Vascular pharmacology·2025

Related Experiment Video

Updated: Jun 5, 2026

Recording of Inward Rectifying K+ Currents in Freshly Isolated Basilar Artery Smooth Muscle Cells by Patch Clamp Technique
07:19

Recording of Inward Rectifying K+ Currents in Freshly Isolated Basilar Artery Smooth Muscle Cells by Patch Clamp Technique

Published on: February 7, 2025

[Decrease of extracellular pH modulates the whole cell voltage-gated potassium currents in rat pulmonary artery

Juan-li Li1, Ming Tang, Ya-qi Duan

  • 1Department of Physiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

Zhongguo Ying Yong Sheng Li Xue Za Zhi = Zhongguo Yingyong Shenglixue Zazhi = Chinese Journal of Applied Physiology
|December 25, 2010
PubMed
Summary

Extracellular acidity reduces potassium currents in pulmonary artery smooth muscle cells. This pH modulation contributes to hypoxic pulmonary vasoconstriction by affecting Kv channels and promoting muscle contraction.

More Related Videos

Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique
08:11

Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique

Published on: November 11, 2022

Microelectrode Impalement Method to Record Membrane Potential from a Cannulated Middle Cerebral Artery
06:32

Microelectrode Impalement Method to Record Membrane Potential from a Cannulated Middle Cerebral Artery

Published on: July 2, 2019

Related Experiment Videos

Last Updated: Jun 5, 2026

Recording of Inward Rectifying K+ Currents in Freshly Isolated Basilar Artery Smooth Muscle Cells by Patch Clamp Technique
07:19

Recording of Inward Rectifying K+ Currents in Freshly Isolated Basilar Artery Smooth Muscle Cells by Patch Clamp Technique

Published on: February 7, 2025

Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique
08:11

Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique

Published on: November 11, 2022

Microelectrode Impalement Method to Record Membrane Potential from a Cannulated Middle Cerebral Artery
06:32

Microelectrode Impalement Method to Record Membrane Potential from a Cannulated Middle Cerebral Artery

Published on: July 2, 2019

Area of Science:

  • Physiology
  • Cardiovascular Research
  • Cellular Electrophysiology

Context:

  • Pulmonary artery smooth muscle cells (PASMCs) play a crucial role in regulating pulmonary vascular tone.
  • Hypoxic pulmonary vasoconstriction (HPV) is a complex physiological response with significant implications for respiratory and cardiovascular health.
  • The precise mechanisms by which extracellular pH influences PASMC function during hypoxia are not fully elucidated.

Purpose:

  • To investigate the impact of varying extracellular pH levels on voltage-gated potassium currents (I(Kv)) in isolated PASMCs.
  • To analyze the electrophysiological characteristics of I(Kv) under acidic conditions.

Summary:

  • Extracellular acidification significantly inhibits I(Kv) in PASMCs, with current density decreasing as pH drops from 7.0 to 6.0.
  • Acidic extracellular pH shifts the current-voltage relationship and alters the voltage-dependence of potassium channel activation, leading to cell depolarization.
  • These findings suggest that extracellular pH changes contribute to the development of HPV by modulating Kv channels, promoting L-type calcium channel opening, and causing PASMC contraction.

Impact:

  • Provides novel insights into the role of extracellular pH in regulating PASMC electrophysiology.
  • Identifies a potential cellular mechanism contributing to the development and progression of hypoxic pulmonary vasoconstriction.
  • Offers a basis for exploring therapeutic strategies targeting pH-dependent ion channel function in pulmonary hypertension.