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Related Experiment Videos

Elephant pituitary gonadotropins.

J R McFarlane1, C M Cabrera, M M Oosthuizen

  • 1Hormone Research Institute, University of California, San Francisco 94143.

General and Comparative Endocrinology
|August 1, 1990
PubMed
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Researchers purified luteinizing hormone (LH) and follicle-stimulating hormone (FSH) from African elephant pituitaries. These purified elephant hormones are similar to ovine hormones and suitable for physiological measurements.

Area of Science:

  • Comparative endocrinology
  • Reproductive biology
  • Elephant physiology

Background:

  • Luteinizing hormone (LH) and follicle-stimulating hormone (FSH) are crucial gonadotropins regulating reproductive functions.
  • Understanding elephant reproductive endocrinology is vital for conservation and management strategies.
  • Previous purification methods for equine and donkey pituitary hormones provide a foundation for elephant hormone isolation.

Purpose of the Study:

  • To describe the first successful purification and characterization of LH and FSH from the African elephant pituitary.
  • To assess the biochemical and biological properties of elephant LH and FSH.
  • To evaluate the potential of these purified hormones for physiological measurements in elephants.

Main Methods:

  • Purification of LH and FSH from anterior pituitary tissue of the African elephant (Loxodonta africana).

Related Experiment Videos

  • Characterization using High-Performance Liquid Chromatography (HPLC) gel filtration and amino acid analysis.
  • Assessment of hormonal activity via radioimmunoassays, bioassays for LH and FSH, and a radioreceptor assay for FSH.

    • Main Results:

    • Purified elephant LH and FSH preparations were obtained with yields of 8.8 mg and 0.48 mg per 10 g pituitary powder, respectively.
    • Biochemical analyses indicated strong similarity between elephant LH/FSH and ovine LH/FSH.
    • Assays confirmed minimal cross-contamination and revealed potencies of elephant LH at 50-66% and FSH at 21-52% compared to ovine standards, with no intrinsic FSH activity in elephant LH.

    Conclusions:

    • The study successfully purified and characterized African elephant LH and FSH, demonstrating their similarity to ovine counterparts.
    • The developed purification and assay methodologies are suitable for physiological measurements of elephant gonadotropins.
    • This research provides essential tools for advancing reproductive physiology studies in African elephants.