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Updated: Jun 5, 2026

Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

Published on: March 17, 2023

Recent advances in plant transformation.

Shyamkumar Barampuram¹, Zhanyuan J Zhang

¹Division of Plant Sciences, University of Missouri, Columbia, MO, USA.

Methods in Molecular Biology (Clifton, N.J.)

|December 25, 2010

Summary

Plant genetic engineering advances crop molecular breeding using efficient DNA delivery methods like Agrobacterium and biolistics. Ongoing research into plant regeneration and transformation promises further improvements in crop biotechnology.

Area of Science:

Plant biotechnology
Crop molecular breeding
Genetic engineering

Background:

Plant genetic engineering is a key tool in modern crop breeding.
Significant advancements in plant transformation methods have occurred in the last decade.
Agrobacterium- and biolistic-mediated transformation are the primary DNA delivery methods.

Purpose of the Study:

To review the progress and current state of plant transformation technologies.
To highlight emerging transgenic-enabling technologies.
To discuss future expectations for plant transformation advancements.

Main Methods:

Review of Agrobacterium-mediated transformation, particularly in cereals and recalcitrant dicots.
Discussion of biolistic-mediated transformation.

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Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Published on: December 16, 2016

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Last Updated: Jun 5, 2026

Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

Published on: March 17, 2023

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants

Published on: December 16, 2016

Exploration of novel technologies like marker-free transgenics, gene targeting, and chromosomal engineering.

Main Results:

Remarkable progress has been achieved in Agrobacterium-mediated transformation of cereals and recalcitrant dicots.
Emergence of advanced techniques such as marker-free transgenics and gene targeting.
Improved understanding of regeneration and transformation mechanisms is driving innovation.

Conclusions:

Plant transformation technologies continue to evolve rapidly.
Further advancements are expected due to a deeper understanding of underlying biological processes.
These improvements will enhance the development of new crop varieties and biotechnologies.