Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 5, 2026

Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)
09:11

Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)

Published on: June 14, 2012

Zinc-finger nucleases: a panoramic view.

Dana Carroll1

  • 1Department of Biochemistry, University of Utah School of Medicine, 15 N. Medical Drive East, Salt Lake City, UT 84112-5650, USA. dana@biochem.utah.edu

Current Gene Therapy
|December 25, 2010
PubMed
Summary

Zinc-finger nucleases (ZFNs) offer precise genome editing capabilities. These engineered DNA-binding proteins enable targeted genetic modifications across various organisms and cell types, with future applications in human therapeutics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rural E-Cigarette Use Higher than Urban Across Age Groups.

Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco·2026
Same author

Knowledge and attitudes about lung cancer screening amongst American Indian adults who use commercial tobacco.

Preventive medicine reports·2025
Same author

Tobacco Product Regulation: Opportunities for Advancing Health Equity in Rural America.

Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco·2025
Same author

Substance Use Screening in Geriatric Primary Care: Cultural Issues and Alcohol Consumption in the Deep South.

Clinical gerontologist·2024
Same author

RNA in Therapeutics: CRISPR in the Clinic.

Molecules and cells·2022
Same author

Centering racial justice for Black/African American and Indigenous American people in commercial tobacco product regulation.

Preventive medicine·2022

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Zinc-finger nucleases (ZFNs) are engineered proteins that facilitate targeted genome modification.
  • ZFNs combine a customizable DNA-binding domain with a DNA-cleaving domain to achieve specificity.

Purpose of the Study:

  • To review the development and applications of ZFNs in genome engineering.
  • To highlight the potential future uses of ZFN technology, particularly in human applications.

Main Methods:

  • Review of scientific literature on ZFN development and applications.
  • Analysis of ZFN mechanisms, including DNA-binding specificity and cleavage activity.

Main Results:

  • ZFNs demonstrate high specificity due to their designed zinc-finger DNA-binding domains and dimerizing cleavage domains.

More Related Videos

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
11:24

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains

Published on: March 25, 2015

Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Related Experiment Videos

Last Updated: Jun 5, 2026

Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)
09:11

Genome Editing with CompoZr Custom Zinc Finger Nucleases (ZFNs)

Published on: June 14, 2012

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
11:24

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains

Published on: March 25, 2015

Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

  • Successful ZFN applications have been reported across diverse organisms and cell types.
  • The technology shows significant promise for future genome engineering strategies.

    • Conclusions:

    • ZFNs are powerful tools for directed genome modification.
    • Their specificity and broad applicability make them valuable for research and potential therapeutic development.
    • Future research is expected to expand ZFN applications, especially in human gene therapy.