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Updated: Jun 5, 2026

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
Published on: April 14, 2016
PCR-based community structure studies of bacteria associated with eukaryotic organisms: a simple PCR strategy to
Ingrid Bakke1, Peter De Schryver, Nico Boon
1Norwegian University of Science and Technology, Department of Biotechnology, Trondheim, Norway. ingrid.bakke@biotech.ntnu.no
Abstract:
PCR primers targeting conserved regions of the SSU rRNA gene are commonly used in bacterial community studies. For microbes associated with eukaryotes, co-amplification of eukaryotic DNA may preclude the analysis. We present a simple and efficient PCR strategy to obtain pure bacterial rDNA amplicons from samples predominated by eukaryotic DNA.
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