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Generation of Porcine Testicular Organoids with Testis Specific Architecture using Microwell Culture
Published on: October 3, 2019
Differential expression of porcine testis proteins during postnatal development
San-Yuan Huang1, Jyh-Hung Lin, Shih-Hua Teng
1Department of Animal Science, National Chung Hsing University, Taiwan. syhuang@dragon.nchu.edu.tw
Animal Reproduction Science
|December 25, 2010
Summary
This study identified key proteins changing during pig testes development, revealing insights into male reproductive maturation and spermatogenesis. Understanding these protein expression patterns is crucial for male reproductive health research.
Area of Science:
- Reproductive Biology
- Proteomics
- Developmental Biology
Background:
- Testicular development involves critical changes in cell structure and hormone levels for male maturation.
- Numerous novel proteins are expressed during testis development, significantly impacting spermatogenesis.
- Systematic studies on differential protein expression during porcine testes development are lacking.
Purpose of the Study:
- To investigate differential protein expression in porcine testes during postnatal development.
- To identify proteins crucial for male reproductive maturation and spermatogenesis in pigs.
Main Methods:
- Proteomic analysis of porcine testes from pigs at 1 week, 3 months, and 1 year of age.
- Quantification of 264 protein spots using Melanie 3 software.
- Identification of differentially expressed proteins using mass spectrometry.
Main Results:
- 108 protein spots exhibited over 2-fold differences (P<0.05) across developmental stages, with 90 identified.
- Proteins showed varied expression trends: 36.1% increased with age, 38.0% decreased, and 25.9% fluctuated.
- Identified proteins were predominantly organelle-associated (41%), involved in protein binding (45%), and cellular/metabolic processes (25%/22%).
Conclusions:
- This research provides a comprehensive proteomic profile of developing porcine testes.
- The identified differentially expressed proteins offer valuable targets for understanding male reproductive development and pathology.
- Findings contribute to the broader knowledge of spermatogenesis and male reproductive system function.
