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Updated: Jun 5, 2026
Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion
Published on: August 15, 2016
Effects of surface treatment and process parameters on immobilization of recombinant yeast cells by adsorption to
Peter Kilonzo1, Argyrios Margaritis, Maurice Bergougnou
1Department of Chemical and Biochemical Engineering, University of Western Ontario, London, Ontario, Canada.
Abstract:
The effects of surface properties of Saccharomyces cerevisiae strains 468/pGAC9 and 468 on adhesion to polyethyleneimine (PEI) and/glutaraldehyde (GA) pre-treated cotton (CT), polyester (PE), polyester+cotton (PECT), nylon (NL), polyurethane foam (PUF), and cellulose re-enforced polyurethane (CPU) fibers were investigated. Process parameters (circulation velocity, pH, ionic strength, media composition and surfactants) were also examined. 80%, 90%, and 35% of the cells were adsorbed onto unmodified CT, PUF, and PE, respectively. PEI-GA pre-treated CT and alkali treated PE yielded 25% and 60% cell adhesion, respectively. Adsorption rate (K(a)) ranged from 0.06 to 0.17 for CT and 0.06-0.16 for PE at varied pH. Adhesion increased by 15% in the presence of ethanol, low pH and ionic strength, and decreased by 23% in the presence of yeast extract and glucose. Shear flow and 1% Triton X-100 detached 62% and 36% nonviable cells from PE and CT, respectively, suggesting that cell immobilization in fibrous-bed bioreactors can be controlled to optimize cell density for long-term stability.
