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Updated: Jun 5, 2026
Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
A simple and inexpensive automated technique for measurement of serum nitrite/nitrate
Etiane Tatsch1, Guilherme Vargas Bochi, Renata da Silva Pereira
1Laboratório de Bioquímica Clínica, Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Objective:
We described an automated technique for measurement of serum nitrite/nitrate (NO(x)) using the Cobas Mira clinical chemistry analyzer.
Design And Methods:
NO(x) was measured by the modified Griess method. Precision, accuracy, linearity, instrument carry-over and lower limit of quantitation (LLOQ) were assessed.
Results:
The automated technique for measurement of serum NO(x) was linear, precise, and accurate. It has a LLOQ of 2.0 μmol/L.
Conclusion:
Serum NO(x) measured by the modified Griess method can be applied easily to the Cobas Mira clinical chemistry analyzer.
