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Updated: Jun 5, 2026
Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
Published on: November 12, 2012
SOE-LRed: A simple and time-efficient method to localize genes with point mutations onto the Escherichia coli
Ryan W Benson1, Tiziana M Cafarelli, Veronica G Godoy
1Department of Biology, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA.
Abstract:
We use a powerful method to replace wild-type genes on the chromosome of Escherichia coli. Using a unique form of PCR, we generate easily constructible gene fusions bearing single point mutations. Used in conjunction with homologous recombination, this method eliminates cloning procedures previously used for this purpose.
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