Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Folate interactions with cerebral G proteins.

D M Hartley1, S R Snodgrass

  • 1Department of Pediatrics, Children's Hospital of Los Angeles 90027.

Neurochemical Research
|July 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA based diagnostic for the quantification of sugarcane root DNA in the field.

Scientific reports·2018
Same author

Some relationships between sociopolitical ideology and moral character among college youth.

Journal of youth and adolescence·2014
Same author

An overview of internet biosurveillance.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2013
Same author

Event-based biosurveillance of respiratory disease in Mexico, 2007-2009: connection to the 2009 influenza A(H1N1) pandemic?

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin·2010
Same author

Repeat instability in the 27-39 CAG range of the HD gene in the Venezuelan kindreds: Counseling implications.

American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics·2008
Same author

Aerosols from insect control measures show dangers of bioterrorism.

Biosecurity and bioterrorism : biodefense strategy, practice, and science·2004

Folic acid and related compounds can overexcite neurons, potentially causing seizures. This study reveals novel mechanisms involving GTP binding and GTPase activity in rat brain membranes, suggesting a new understanding of folate neurotoxicity.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Pharmacology

Background:

  • Intracerebral folate administration causes seizures and brain lesions.
  • Folates are known to excite neurons through poorly understood mechanisms.
  • Folates modulate GTP binding and GTPase activity in non-mammalian organisms.

Purpose of the Study:

  • To investigate the molecular mechanisms underlying folate-induced neuroexcitation and toxicity in rat brain membranes.
  • To determine if folates interact with G protein-coupled receptors (GPCRs) or related signaling pathways.

Main Methods:

  • Incubation of rat brain membranes (cerebellar and hippocampal) with various folate compounds (folic acid, DHF, THF, 5-methyl-THF).
  • Measurement of guanosine triphosphate (GTP) analog (GTPγS) binding and high-affinity GTPase activity.

Related Experiment Videos

  • Analysis of [3H]folic acid binding to membrane receptors and release of [3H]GDP.

    • Main Results:

    • Folic acid, DHF, and THF significantly stimulated GTPγS binding and inhibited GTPase activity in a concentration-dependent manner, with effects most pronounced in cerebellar and hippocampal membranes.
    • 5-methyltetrahydrofolate was less potent than other folates.
    • High-affinity, guanine nucleotide-sensitive binding of [3H]folic acid and stimulation of [3H]GDP release were observed, indicative of GPCR-like interactions.

    Conclusions:

    • Folates exert neurotoxic and neuroexcitatory effects through direct interaction with brain membrane proteins, modulating GTP binding and GTPase activity.
    • These interactions, distinct from known metabolic functions, resemble G protein-coupled receptor signaling and may explain folate-induced neurotoxicity.
    • The findings suggest a novel mechanism for folate neurotoxicity relevant to neurological conditions.