Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peroxisomes01:24

Peroxisomes

Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
Peroxisomes01:24

Peroxisomes

Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
Peroxisomes01:30

Peroxisomes

Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within peroxisomes...
Protein Import into the Peroxisomes01:27

Protein Import into the Peroxisomes

Cells contain membrane-bound organelles called peroxisomes that oxidize organic molecules by transferring hydrogen atoms to oxygen, producing hydrogen peroxide. Peroxisomes enzymatically convert the released hydrogen peroxide into water and oxygen.
Peroxisomal Protein Import:
Peroxisomes lack the genetic machinery required to code for their own proteins. Hence, most peroxisomal membrane, lumenal and transmembrane proteins are synthesized in the cytoplasm or ER and transported to the peroxisome...
Porin Insertion in the Outer Mitochondrial Membrane01:12

Porin Insertion in the Outer Mitochondrial Membrane

Porins are beta-barrel proteins translocated to the mitochondrial outer membrane through the TOM complex into the intermembrane space. Porin precursors bind TIM chaperones within the intermembrane space and are guided to the Sorting and Assembly Machinery complex or SAM complex on the outer mitochondrial membrane.
Three models describe the assembly of porins by the SAM complex and their insertion into the outer membrane. Model 1 suggests that porins are assembled outside the SAM channel as the...
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Host oxidative stress primes mycobacteria for rapid antibiotic resistance evolution.

Nature communications·2026
Same author

Pex3p phosphorylation modulates recruitment of myosin V adapters Inp2p and Pex19p to regulate peroxisome partitioning in yeast.

Molecular biology of the cell·2026
Same author

Updated consensus guidelines for the diagnosis and management of patients with HCL and HCL variant.

Blood·2026
Same author

High-Resolution Quantification of Two-Way Nanobody Synergy Using Automated Liquid Handling and Computational Modeling.

Bio-protocol·2026
Same author

Host oxidative stress primes mycobacteria for rapid antibiotic resistance evolution.

bioRxiv : the preprint server for biology·2025
Same author

TOR and heat shock response pathways regulate peroxisome biogenesis during proteotoxic stress.

Nature communications·2025

Related Experiment Video

Updated: Jun 5, 2026

Peroxisome Staining in Mammalian Cells Using Peroxisome-Specific Probes
05:57

Peroxisome Staining in Mammalian Cells Using Peroxisome-Specific Probes

Published on: December 19, 2025

Peroxisome biogenesis: something old, something new, something borrowed.

Fred D Mast1, Andrei Fagarasanu, Barbara Knoblach

  • 1Department of Cell Biology, University of Alberta, Edmonton, Alberta, Canada.

Physiology (Bethesda, Md.)
|December 28, 2010
PubMed
Summary

This review explores the role of peroxisomes in regulating lipid metabolism through oxidative reactions. Peroxisomes are membrane-bound organelles that may be essential for lipid availability and cellular differentiation. The study synthesizes evidence from the literature to clarify how peroxisomes contribute to metabolic processes. The findings suggest that peroxisomes may play a role in organismal development and lipid homeostasis. The review highlights the need for further research on peroxisome biogenesis and function.

Keywords:
peroxisome functionlipid homeostasiscellular metabolismorganelle biogenesis

Frequently Asked Questions

More Related Videos

Monitoring Stub1-Mediated Pexophagy
08:26

Monitoring Stub1-Mediated Pexophagy

Published on: May 12, 2023

Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome
10:04

Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome

Published on: August 19, 2014

Related Experiment Videos

Last Updated: Jun 5, 2026

Peroxisome Staining in Mammalian Cells Using Peroxisome-Specific Probes
05:57

Peroxisome Staining in Mammalian Cells Using Peroxisome-Specific Probes

Published on: December 19, 2025

Monitoring Stub1-Mediated Pexophagy
08:26

Monitoring Stub1-Mediated Pexophagy

Published on: May 12, 2023

Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome
10:04

Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome

Published on: August 19, 2014

Area of Science:

  • Cell biology
  • Metabolic pathways
  • Membrane biology

Background:

Peroxisomes are membrane-bound organelles found in eukaryotic cells. Their role in lipid metabolism is well established. Prior research has shown that peroxisomes are involved in oxidative reactions that regulate lipid availability. However, the mechanisms underlying peroxisome biogenesis remain unclear. This gap motivated recent investigations into how these organelles form and function. No prior work had resolved the full scope of peroxisome biogenesis. The study of peroxisomes has been limited by a lack of detailed mechanistic insights. Understanding peroxisome biogenesis could provide new perspectives on cellular metabolism. The need for clarity on peroxisome formation is evident in current scientific discourse.

Purpose Of The Study:

This study aimed to clarify the mechanisms of peroxisome biogenesis. The specific problem addressed is the incomplete understanding of how peroxisomes form and function. The motivation stems from the need to connect peroxisome biogenesis to broader metabolic processes. The study sought to explore the role of oxidative reactions in peroxisome function. Understanding these processes could reveal new insights into cellular differentiation. The research focused on the synthesis and availability of lipids. The goal was to synthesize existing knowledge with new findings. This approach may help bridge gaps in peroxisome biology.

Main Methods:

The study employed a review approach to synthesize evidence from existing literature. Key findings were extracted from peer-reviewed articles on peroxisome biogenesis. The researchers analyzed data on oxidative reactions and lipid metabolism. They examined how peroxisomes contribute to cellular differentiation. The literature was categorized based on function and structure. Comparative analysis was used to identify common themes. The synthesis focused on the role of peroxisomes in metabolic processes. The review approach allowed for a comprehensive overview of peroxisome biology.

Main Results:

The literature suggests that peroxisomes regulate lipid synthesis through oxidative reactions. Key findings from the literature indicate that peroxisomes are essential for lipid availability. The review highlights the role of peroxisomes in cellular differentiation. Peroxisomes contribute to organismal development through metabolic processes. The synthesis of evidence shows that peroxisomes are involved in diverse metabolic pathways. The findings suggest that peroxisomes may play a role in regulating lipid homeostasis. The review proposes that peroxisomes are important for maintaining cellular function. These results may inform future research on peroxisome biology.

Conclusions:

The synthesis of evidence suggests that peroxisomes are involved in lipid regulation and metabolic processes. The literature indicates that peroxisomes contribute to cellular differentiation. The findings may help clarify the mechanisms of peroxisome biogenesis. The review approach allowed for a comprehensive overview of peroxisome function. The results suggest that peroxisomes may be important for organismal development. The study highlights the need for further research on peroxisome biogenesis. The conclusions are based on the authors' stated implications. These findings may inform future studies on peroxisome biology.

The authors propose that peroxisomes regulate lipid synthesis through oxidative reactions, which influence lipid availability and homeostasis.

The literature suggests that peroxisomes participate in metabolic processes essential for cellular differentiation, though specific mechanisms remain unclear.

Oxidative reactions in peroxisomes may be necessary for lipid regulation, which is central to peroxisome function and metabolic processes.

The review suggests that peroxisomes contribute to organismal development through their involvement in metabolic pathways and lipid homeostasis.

Lipid availability is linked to peroxisome function through oxidative reactions, which may regulate lipid synthesis and cellular metabolism.

The authors suggest that these findings may inform future studies on peroxisome biogenesis and their role in cellular and organismal processes.