Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bacterial Phylum Bacteroidota01:26

Bacterial Phylum Bacteroidota

The phylum Bacteroidota includes over 700 species classified into four primary orders: Bacteroidales, Cytophagales, Flavobacteriales, and Sphingobacteriales. These gram-negative, non-sporulating rods exhibit saccharolytic capabilities and can be aerobic or fermentative, encompassing obligate aerobes, facultative aerobes, and obligate anaerobes. Many species display gliding motility, though some are nonmotile or use flagella. The genus Bacteroides is well-studied due to its significant role in...
Bacterial Phylum Verrucomicrobiota01:26

Bacterial Phylum Verrucomicrobiota

The phylum Verrucomicrobiota comprises at least four characterized orders, with most species classified within the order Verrucomicrobiotales. Members of this phylum are either aerobic or facultatively aerobic, with the ability to ferment sugars. A notable exception is the genus Methylacidiphilum, which consists of aerobic methanotrophs. Additionally, some Verrucomicrobiota establish symbiotic relationships with protists. These bacteria are widely distributed across various environments,...
Bacterial Phylum Actinobacteria01:30

Bacterial Phylum Actinobacteria

Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
Bacterial Flora of the Large Intestine01:29

Bacterial Flora of the Large Intestine

The gut microbiome is formed by a vast and diverse community of bacteria that colonizes our large intestine. These bacteria start residing in the gut from birth and continue diversifying throughout life, influenced by factors such as diet, lifestyle, and stress. The gut bacterial community also includes bacteria from food and those that enter the colon through the anus.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
Anoxygenic Phototrophic Bacteria01:28

Anoxygenic Phototrophic Bacteria

Anoxygenic phototrophic bacteria are a diverse group of microorganisms that perform photosynthesis without producing oxygen. They primarily include purple sulfur bacteria, purple nonsulfur bacteria, green sulfur bacteria, and green nonsulfur bacteria. These bacteria are classified into the Gammaproteobacteria, Alphaproteobacteria, Betaproteobacteria, Chlorobi, and Chloroflexi lineages, each with distinct physiological and ecological adaptations.Purple sulfur bacteria belong to the...
iChip01:24

iChip

The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Factors affecting health-related quality of life in cervical cancer survivors: a multicenter cross-sectional study in Korea.

BMC women's health·2026
Same author

Prognostic significance of psoas muscle index at diagnosis in cervical cancer progression.

Journal of gynecologic oncology·2026
Same author

Diagnostic accuracy of the droplet digital PCR POLE mutation test in endometrial cancer: comparison with Sanger sequencing and NGS.

Journal of gynecologic oncology·2026
Same author

Genomic Insights into Ciprofloxacin-Resistant Enteropathogenic <i>Escherichia coli</i> ST752 in Republic of Korea: A One Health Perspective on Its Emergence and Transmission.

Antibiotics (Basel, Switzerland)·2026
Same author

Clinical practice guideline for high-risk human papillomavirus testing in cervical cancer screening: a consensus statement from the Korean Society of Gynecologic Oncology.

Journal of gynecologic oncology·2026
Same author

Hypoosmolarity inhibits ammonia oxidation by terrestrial and freshwater Nitrosopumilaceae members.

The ISME journal·2026

Related Experiment Video

Updated: Jun 5, 2026

In Situ Isolation and Culturing of Recalcitrant Soil Bacteria using an Isolation Chip (iChip)
11:07

In Situ Isolation and Culturing of Recalcitrant Soil Bacteria using an Isolation Chip (iChip)

Published on: August 6, 2025

Flavobacterium chungbukense sp. nov., isolated from soil.

Chae-Sung Lim1, Yong-Sik Oh1, Jae-Kwan Lee1

  • 1Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.

International Journal of Systematic and Evolutionary Microbiology
|December 28, 2010
PubMed
Summary

A novel bacterium, Flavobacterium chungbukense sp. nov., was discovered in Korean soil. This yellow-pigmented organism is a new species within the Flavobacterium genus, expanding our understanding of soil microbial diversity.

More Related Videos

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
10:33

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Published on: May 27, 2022

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
06:34

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

Related Experiment Videos

Last Updated: Jun 5, 2026

In Situ Isolation and Culturing of Recalcitrant Soil Bacteria using an Isolation Chip (iChip)
11:07

In Situ Isolation and Culturing of Recalcitrant Soil Bacteria using an Isolation Chip (iChip)

Published on: August 6, 2025

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
10:33

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Published on: May 27, 2022

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
06:34

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

Area of Science:

  • Microbiology
  • Bacteriology
  • Environmental Microbiology

Background:

  • The genus Flavobacterium comprises diverse, Gram-negative bacteria found in various environments.
  • Characterization of novel bacterial species is crucial for understanding microbial ecology and evolution.
  • Soil ecosystems harbor a vast, largely uncharacterized microbial diversity.

Purpose of the Study:

  • To isolate and characterize a novel bacterial strain from Korean soil.
  • To determine the taxonomic position of the novel isolate using phylogenetic and chemotaxonomic analyses.
  • To propose a new species designation for the characterized bacterium.

Main Methods:

  • Isolation and cultivation of bacteria from soil samples.
  • Morphological, physiological, and biochemical characterization of the isolate (strain CS100(T)).
  • Phylogenetic analysis based on 16S rRNA gene sequencing.
  • Chemotaxonomic analyses including fatty acid profiling and respiratory quinone determination.
  • DNA-DNA hybridization for relatedness studies.

Main Results:

  • Strain CS100(T) is a yellow-pigmented, Gram-negative, aerobic rod, producing flexirubin-type pigments.
  • Phylogenetic analysis placed strain CS100(T) within the genus Flavobacterium, with highest 16S rRNA gene similarity to Flavobacterium glaciei (97.6%) and Flavobacterium johnsoniae (97.1%).
  • Physiological tests revealed growth at 4–30 °C, pH 5.0–9.0, and 0–2% NaCl, with positive oxidase and catalase activities.
  • Chemotaxonomic data showed MK-6 as the major respiratory quinone and specific major fatty acids.
  • Low DNA-DNA relatedness values (3.0% with F. glaciei and 11.5% with F. johnsoniae) confirmed its distinctiveness.

Conclusions:

  • Strain CS100(T) represents a novel species of the genus Flavobacterium based on polyphasic taxonomic data.
  • The proposed name for this new species is Flavobacterium chungbukense sp. nov.
  • The type strain for this novel species is CS100(T) (KACC 15048(T) = JCM 17386(T)).