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[The advantages and disadvantages of GMS and PAS Staining in fungal keratitis]
Jianliang Zheng1, Wenxin Zhang, Jianxian Lin
1State Key Laboratory of Ophthalmology,Pathology laboratory,Zhongshan Ophthalmic Center,Sun Yat-sen University,Guangzhou 510060,China.
Purpose:
To compare the advantages and disadvantages of GMS and PAS Staining in the diagnosis of fungal keratitis.
Methods:
Retrospectively analysed the sections of 102 cases of fungal keratitis from November 2007 to June 2010 in our department. Statistically analysed the positive rate of different statining methods, and summarized technical cruxes of GMS and PAS staining.
Results:
The positive rates of GMS and PAS staining for fungal keratitis were 100% and 93.14%, respectively.
Conclusion:
GMS staining is better than PAS staining for fungal keratitis in displaying fungi. And it takes less time, is easier to operate and presents a more durable colour compared with PAS staining. But PAS staining is superior to GMS staining in displaying fungal septa and eliminating interference from pigment-containing cells and other cells.
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