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Updated: Jun 5, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

[Staphylococcus aureus: new and old antimicrobial agents].

B Perazzi¹, M Camacho, K Bombicino

¹Laboratorio de Bacteriología Clinica, Departamento de Bioqufmica Clinica, Hospital de Clinicas, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires. Av. Córdoba 2351 (1120) Ciudad Autónoma de Buenos Aires. hugodandrea@ciudad.com.ar

Revista Argentina De Microbiologia

|December 29, 2010

Summary

This summary is machine-generated.

Hospital-acquired oxacillin-resistant Staphylococcus aureus (HA-ORSA) shows resistance to many agents, but remains susceptible to tigecycline and linezolid. Community-acquired ORSA (CA-ORSA) is susceptible to most agents, indicating distinct resistance profiles.

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Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

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Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Published on: February 14, 2025

Area of Science:

Clinical Microbiology
Infectious Diseases
Antimicrobial Resistance

Background:

Staphylococcus aureus is a significant pathogen causing both hospital-acquired (HA-ORSA) and community-acquired (CA-ORSA) infections.
Understanding antimicrobial susceptibility patterns is crucial for effective treatment of S. aureus infections.
Oxacillin resistance (ORSA) necessitates evaluation of both established and novel antimicrobial agents.

Purpose of the Study:

To assess the in vitro susceptibility of HA-ORSA, CA-ORSA, and oxacillin-susceptible S. aureus (OSSA) to various old and new antimicrobial agents.
To characterize genetic markers associated with ORSA phenotypes, including the mecA gene and Panton-Valentine leukocidin (PVL).

Main Methods:

Agar dilution method following Clinical and Laboratory Standards Institute (CLSI) guidelines was used to determine minimum inhibitory concentrations (MICs).
Polymerase chain reaction (PCR) was employed to detect specific resistance genes (mecA, PVL, gamma-hemolysin) and SCCmec cassette types in ORSA isolates.
118 consecutive S. aureus isolates (44 HA-ORSA, 16 CA-ORSA, 58 OSSA) were analyzed.

Main Results:

HA-ORSA isolates exhibited multidrug resistance, with susceptibility only to tigecycline (TIG), vancomycin, teicoplanin, and linezolid (LZD).
CA-ORSA isolates were susceptible to all tested non-oxacillin agents, with resistance primarily to oxacillin.
All CA-ORSA isolates carried the mecA, PVL, gamma-hemolysin genes, and SCCmec type IV, distinguishing them from HA-ORSA.

Conclusions:

HA-ORSA demonstrates extensive resistance, highlighting the importance of glycopeptides, doxycycline, rifampin, trimethoprim-sulfamethoxazole, LZD, and TIG for treatment.
CA-ORSA retains susceptibility to a broad range of agents, with the SCCmec type IV being a strong indicator.
Distinct antimicrobial susceptibility profiles between HA-ORSA and CA-ORSA underscore the need for targeted therapeutic strategies.