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Related Experiment Videos

A screening test for detecting sulfonylureas in plasma.

G M Shenfield1, J S Boutagy, C Webb

  • 1Department of Clinical Pharmacology, Royal North Shore Hospital, Sydney, Australia.

Therapeutic Drug Monitoring
|July 1, 1990
PubMed
Summary

Distinguishing hypoglycemia from sulfonylurea ingestion is challenging. A new HPLC method reliably detects common sulfonylurea drugs in plasma, aiding diagnosis.

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Q methodology.

Nurse researcher·2016

Area of Science:

  • Clinical Chemistry
  • Analytical Chemistry
  • Pharmacology

Background:

  • Surreptitious sulfonylurea ingestion can mimic insulinomas, complicating hypoglycemia diagnosis.
  • Accurate identification of the causative agent is crucial for effective patient management.

Observation:

  • A novel technique using high-performance liquid chromatography (HPLC) was developed for sulfonylurea detection.
  • The method involves plasma acidification, ether extraction, and HPLC analysis with UV detection at 230 nm.

Findings:

  • The HPLC assay effectively separates and detects five common sulfonylurea drugs: chlorpropamide, glibenclamide, gliclazide, glipizide, and tolbutamide.
  • The assay is sensitive enough to identify clinically relevant concentrations, providing semiquantitative results.
  • The screening test demonstrated diagnostic value in four reported case histories.

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Implications:

  • This rapid and reliable screening test can significantly aid in diagnosing hypoglycemia of uncertain origin.
  • Early and accurate diagnosis facilitates appropriate treatment and management of patients with sulfonylurea-induced hypoglycemia.