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Identification and interpretation of longitudinal gene expression changes in trauma
Natasa Rajicic1, Joseph Cuschieri, Dianne M Finkelstein
1Pfizer Inc., New York, New York, United States of America. Natasa.Rajicic@pfizer.com
Plos One
|December 29, 2010
Summary
Leukocyte gene expression after blunt trauma predicts respiratory recovery. Specific gene networks and pathways, including antigen processing and JAK-signaling, are associated with patient outcomes following injury.
Area of Science:
- Genomics
- Immunology
- Trauma Research
Background:
- Leukocyte gene expression following injury may elucidate the mechanisms of multiple organ dysfunction.
- Understanding these relationships can inform strategies for improving patient recovery.
Purpose of the Study:
- Identify genes whose expression predicts respiratory recovery post-injury.
- Characterize the biological networks and pathways associated with these predictive genes.
Main Methods:
- Collected blood samples from 147 blunt trauma patients over 28 days.
- Measured leukocyte gene expression using oligonucleotide arrays.
- Employed statistical models to identify gene expression trajectories predicting respiratory recovery.
Main Results:
- Identified 135 genes (170 probe-sets) related to respiratory recovery.
- Genes mapped to nine biological networks.
- Activated pathways included antigen processing and presentation, and JAK-signaling.
Conclusions:
- Gene expression patterns over time correlate with clinical recovery after severe injury.
- This approach may reveal mechanisms underlying recovery or its absence.
