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Updated: Jun 5, 2026

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry
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Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Published on: May 21, 2018

1H, 13C and 15N assignment of the GNA1946 outer membrane lipoprotein from Neisseria meningitidis

A Neumoin1, A Leonchiks, P Petit

  • 1Bijvoet Center for Biomolecular Research, Science Faculty, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.

Biomolecular NMR Assignments
|December 29, 2010
PubMed

Insights

Researchers have assigned nuclear magnetic resonance signals for Genome-derived Neisseria Antigen 1946 (GNA1946), a key protein from Neisseria meningitidis. This structural data in solution aids in developing new vaccines against bacterial meningitis.

Area of Science:

  • Structural biology
  • Protein chemistry
  • Vaccine development

Background:

  • Neisseria meningitidis serogroup B causes severe meningitis.
  • GNA1946 is a conserved outer membrane lipoprotein antigen of N. meningitidis.
  • Understanding GNA1946's solution behavior is crucial for vaccine design.

Purpose of the Study:

  • To provide complete resonance assignments for GNA1946 in aqueous solution.
  • To facilitate the identification of antigenic determinants on GNA1946.
  • To support the development of a meningococcal serogroup B vaccine.

Main Methods:

  • Nuclear Magnetic Resonance (NMR) spectroscopy.
  • Collected (1)H, (13)C, and (15)N resonance assignments.
  • Studied GNA1946 (residues 10-287) in aqueous buffer.

Main Results:

  • Achieved near-complete (1)H, (13)C, and (15)N resonance assignments for GNA1946.
  • Provided foundational data on GNA1946 structure and dynamics in solution.
  • Enabled further investigation into GNA1946's antigenic properties.

Conclusions:

  • NMR assignments provide critical insights into GNA1946's solution structure.
  • This data is essential for epitope mapping and rational vaccine design.
  • Facilitates progress towards an effective N. meningitidis serogroup B vaccine.

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