The effects of hypertonic glucose solutions on hepatic bile formation

Bile secretion in the isolated guinea-pig liver was studied during perfusion with equi-osmolar hypertonic solutions containing either glucose, galactose, mannose, mannitol or sodium chloride. Perfusates made hypertonic with glucose, galactose or mannose decreased bile flow to the same extent and had similar effects on the ionic composition of bile: sodium, potassium and bicarbonate concentrations all increased. Mannitol had a smaller inhibitory effect and caused different changes in ionic composition: the increase in bile potassium concentration was proportionately greater; bicarbonate concentration did not change, but chloride was increased. Thus, glucose, galactose and mannose, can inhibit bile flow independently of extrinsic neural and hormonal mechanisms and exert a greater cholestatic effect than a non-metabolisable carbohydrate of similar molecular weight. The results also provide evidence for glucose reabsorption in the guinea-pig biliary tree, as shown in other species, and that galactose competes for this transport.