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Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
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Published on: October 2, 2017

Sialic acid transport contributes to pneumococcal colonization.

Carolyn Marion1, Amanda M Burnaugh, Shireen A Woodiga

  • 1Center for Microbial Pathogenesis, The Research Institute at Nationwide Children’s Hospital, Columbus, OH 43205-2696, USA.

Infection and Immunity
|December 30, 2010
PubMed
Summary
This summary is machine-generated.

Streptococcus pneumoniae uses sialic acid as a vital carbon source for growth and colonization. A specific transporter, SatABC, is crucial for this process, impacting bacterial survival in the respiratory tract.

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Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Molecular Biology

Background:

  • Streptococcus pneumoniae causes pneumonia and meningitis, requiring airway colonization.
  • Carbohydrates are essential for bacterial growth, but readily available sources are scarce in the airway.
  • N- and O-linked glycans, abundant in airways, are modified with sialic acid, a potential nutrient source.

Purpose of the Study:

  • To investigate if Streptococcus pneumoniae can utilize sialic acid as a sole carbon source.
  • To identify the transport mechanism for sialic acid in S. pneumoniae.
  • To determine the role of sialic acid utilization in pneumococcal colonization.

Main Methods:

  • Culturing S. pneumoniae with sialic acid as the sole carbon source.
  • Genetic analysis to identify and characterize sialic acid transporters.
  • Construction and testing of a satABC mutant for growth and colonization.
  • In vivo colonization assays in a murine upper respiratory tract model.

Main Results:

  • Sialic acid supports S. pneumoniae growth as a sole carbon source.
  • The satABC transporter is essential for sialic acid import and utilization.
  • A satABC mutant exhibits significantly reduced growth on sialic acid and human glycoprotein alpha-1.
  • The satABC mutant shows a marked decrease in upper respiratory tract colonization in mice.

Conclusions:

  • Streptococcus pneumoniae can metabolize sialic acid for growth.
  • The satABC transporter plays a critical role in sialic acid uptake and is important for colonization.
  • Sialic acid utilization is a key factor in pneumococcal pathogenesis and survival in vivo.