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Related Experiment Videos

Fish apolipoprotein-A-I has heparin binding activity: implication for nerve regeneration.

A Harel1, M Fainaru, M Rubinstein

  • 1Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel.

Journal of Neurochemistry
|October 1, 1990
PubMed
Summary

Fish apolipoprotein-A-I (apo-A-I) from plasma and nerves binds to heparin. This heparin binding may facilitate apo-A-I accumulation in fish optic nerves after injury.

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Area of Science:

  • Neuroscience
  • Biochemistry
  • Molecular Biology

Background:

  • Injury to the fish central nervous system (CNS), specifically optic nerves, increases apolipoprotein-A-I (apo-A-I) levels.
  • Apolipoprotein-A-I is found in both fish plasma and nerve tissues.

Purpose of the Study:

  • To purify and characterize apolipoprotein-A-I (apo-A-I) from fish plasma and optic nerves.
  • To investigate the heparin binding activity of purified fish apo-A-I.

Main Methods:

  • Purification of apo-A-I from fish plasma and optic nerves.
  • N-terminal amino acid sequence analysis.
  • Heparin binding assays using radiolabeled heparin and Scatchard analysis.

Main Results:

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  • Purified apo-A-I from both sources showed high sequence identity (at least 14 out of 15 N-terminal amino acids).
  • Fish apo-A-I demonstrated heparin binding activity with a dissociation constant (KD) of 2.8 x 10(-6) M.
  • This binding indicates a relatively low affinity interaction.

    • Conclusions:

    • Fish apo-A-I possesses heparin binding capabilities.
    • Heparin binding may mediate the accumulation of apo-A-I in the extracellular matrix of fish optic nerves following injury.
    • This mechanism is analogous to apolipoprotein-E binding in mammals.