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Updated: Jun 5, 2026

Closed System Cell Culture Protocol Using HYPERStack Vessels with Gas Permeable Material Technology

Closed System Cell Culture Protocol Using HYPERStack Vessels with Gas Permeable Material Technology

Published on: November 29, 2010

Closed system cell culture protocol using HYPERStack vessels with gas permeable material technology.

Kim Titus¹, Vitaly Klimovich, Mark Rothenberg

¹Business Development, Corning Life Science. titusk@corning.com

Journal of Visualized Experiments : Jove

|December 30, 2010

Summary

HYPERStack vessels offer a closed system for large-scale adherent cell culture, significantly increasing cell yield by over 2.5X within the same footprint using gas-permeable technology.

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Area of Science:

Biotechnology
Cell Culture Technology
Bioprocessing

Background:

Standard adherent cell culture often relies on stacked plates, which can be suboptimal for large volumes.
Microcarrier beads are not always suitable for all cell culture applications.
Existing methods lack efficient closed-system scale-up capabilities.

Purpose of the Study:

To introduce HYPERStack vessels as an advancement in large-volume adherent cell culture.
To demonstrate the closed-system capabilities and scalability of HYPERStack vessels.
To detail the technology and protocols for utilizing HYPERStack vessels in bioproduction.

Main Methods:

Utilizing HYPERStack vessels with gas-permeable materials for enhanced gas exchange.
Implementing closed-system protocols for cell and reagent manipulation.

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Last Updated: Jun 5, 2026

Closed System Cell Culture Protocol Using HYPERStack Vessels with Gas Permeable Material Technology

Closed System Cell Culture Protocol Using HYPERStack Vessels with Gas Permeable Material Technology

Published on: November 29, 2010

Permeabilization of Adhered Cells Using an Inert Gas Jet

Permeabilization of Adhered Cells Using an Inert Gas Jet

Published on: September 4, 2013

Large-Scale Cell Production Based on GMP-Grade Dissolvable Porous Microcarriers

Large-Scale Cell Production Based on GMP-Grade Dissolvable Porous Microcarriers

Published on: July 7, 2023

Evaluating gas diffusion rates to meet cellular metabolic demands.

Main Results:

HYPERStack vessels achieve over 2.5 times more cells within the same volumetric footprint compared to traditional stacked plates.
The gas-permeable material eliminates the need for internal headspace, enabling a minimized cell growth compartment.
Successful closed-system operation for cell growth and harvesting was demonstrated.

Conclusions:

HYPERStack vessels provide a scalable, closed-system solution for large-volume adherent cell culture.
This technology optimizes space utilization and increases cell production efficiency.
The system is suitable for critical applications like cell therapy and vaccine production.