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Updated: Jun 5, 2026

Bioenergetic Profile Experiment using C2C12 Myoblast Cells
07:20

Bioenergetic Profile Experiment using C2C12 Myoblast Cells

Published on: December 6, 2010

Bioenergetic profile experiment using C2C12 myoblast cells.

David G Nicholls1, Victor M Darley-Usmar, Min Wu

  • 1Buck Institute for Age Research, Novato, CA, USA.

Journal of Visualized Experiments : Jove
|December 30, 2010
PubMed
Summary
This summary is machine-generated.

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This study measures cellular metabolism using oxygen consumption (OCR) and extracellular acidification (ECAR) rates in C2C12 myoblasts. The findings provide a framework for understanding mitochondrial function in various diseases.

Area of Science:

  • Cellular and Molecular Biology
  • Biochemistry
  • Physiology

Background:

  • Cellular metabolism involves substrate uptake and energy conversion, producing ATP, heat, and byproducts like lactate and CO2.
  • Mitochondrial dysfunction is implicated in obesity, diabetes, aging, cancer, cardiovascular disease, and toxicity.
  • Measuring oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) offers insight into cellular bioenergetics.

Purpose of the Study:

  • To establish baseline rates of oxygen consumption (OCR) and extracellular acidification (ECAR) in C2C12 myoblast cells.
  • To investigate the metabolic response of cells to perturbations using a Seahorse assay.
  • To provide a framework for complex experiments assessing mitochondrial function and cellular response to stress.

Main Methods:

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  • C2C12 myoblast cells were cultured in Seahorse plates.
  • Basal OCR and ECAR were measured to establish baseline cellular metabolism.
  • Cells were subsequently treated with sequential additions of compounds to perturb their bioenergetic profile.

Main Results:

  • Baseline OCR and ECAR were successfully measured in C2C12 myoblasts.
  • Metabolic perturbations induced measurable shifts in the cells' bioenergetic profiles.
  • The assay demonstrated the ability to assess cellular response to metabolic challenges.

Conclusions:

  • The Seahorse assay provides a robust method for evaluating cellular metabolism and mitochondrial function.
  • This approach allows for the assessment of cellular responses to various metabolic conditions and potential insults.
  • Understanding cellular bioenergetics is crucial for research into metabolic diseases and cellular health.