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Protein Dynamics in Living Cells

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Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)

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Integrin-targeting block copolymer probes for two-photon fluorescence bioimaging.

Sanchita Biswas¹, Xuhua Wang, Alma R Morales

¹Department of Chemistry, The College of Optics and Photonics, University of Central Florida, Orlando, Florida 32816, USA.

Biomacromolecules

|December 31, 2010

Summary

Researchers developed a novel polymer probe for targeted molecular imaging using two-photon fluorescence microscopy (2PFM). This biocompatible scaffold selectively targets cancer cells overexpressing α(v)β(3) integrin, showing promise for disease diagnosis and treatment.

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Area of Science:

Biomaterials Science
Molecular Imaging
Chemical Biology

Background:

Targeted molecular imaging with two-photon fluorescence microscopy (2PFM) offers potential for disease diagnosis and treatment.
Developing versatile scaffolds for advanced bioimaging is crucial in chemical biology.

Purpose of the Study:

To synthesize and characterize a novel norbornene-based block copolymer multifunctional scaffold.
To evaluate the scaffold's potential for targeted molecular imaging and integrin-targeting in cancer cells.

Main Methods:

Synthesis and photophysical studies of a polymer probe with PEG, fluorenyl dyes, and cyclic-RGD peptide.
Characterization of self-assembled structures using transmission electron microscopy (TEM) and dynamic light scattering (DLS).
Cell viability assays and two-photon fluorescence microscopy (2PFM) imaging on U87MG and MCF-7 cell lines.

Main Results:

The polymer probe self-assembled into spherical micelles in water, with sizes determined by TEM and DLS.
2PFM imaging demonstrated high selectivity for integrin-rich U87MG cells.
Blocking and negative control experiments confirmed the probe's specific integrin-targeting ability.

Conclusions:

The developed biocompatible polymer probe is effective for targeted molecular imaging.
The probe shows high specificity for α(v)β(3) integrin, indicating potential for noninvasive diagnosis and treatment of related diseases.