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Temporal resolution in electrochemical imaging on single PC12 cells using amperometry and voltammetry at
Bo Zhang1, Michael L A V Heien, Michael F Santillo
1Department of Chemistry, University of Washington, Seattle, Washington 98195, USA.
Analytical Chemistry
|December 31, 2010
Summary
Carbon-fiber microelectrode arrays (MEAs) enable electrochemical imaging of dopamine release from PC12 cells. Comparing amperometry and fast-scan cyclic voltammetry (FSCV) reveals trade-offs in spatial and temporal resolution due to molecular diffusion.
Area of Science:
- Neuroscience
- Electrochemistry
- Cell Biology
Background:
- Carbon-fiber microelectrode arrays (MEAs) are used for electrochemical imaging of neurochemical secretion.
- Pheochromocytoma (PC12) cells are a common model for studying neurotransmitter release.
Purpose of the Study:
- To electrochemically image dopamine release from individual PC12 cells.
- To quantitatively compare the spatial and temporal resolution of amperometry and fast-scan cyclic voltammetry (FSCV).
Main Methods:
- Utilized carbon-fiber microelectrode arrays (MEAs) for electrochemical monitoring.
- Employed alternately constant-potential amperometry and fast-scan cyclic voltammetry (FSCV).
- Developed numerical simulations of molecular diffusion and flux at the electrode-cell interface.
Main Results:
- Dopamine release events were monitored at seven locations on single PC12 cells.
- FSCV offers superior chemical resolution but compromised spatial and temporal resolution compared to amperometry.
- Numerical simulations explained differences in resolution and signal crosstalk based on molecular diffusion and electrode potential.
Conclusions:
- Molecular diffusion and electrochemical reactions significantly impact temporal resolution in electrochemical imaging.
- Differences in diffusion and electrode potential explain signal crosstalk variations between amperometry and FSCV.
