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[Fetal RHD genotyping by PCR using plasma from D negative pregnant women]
S Achargui1, M Tijane, N Benchemsi
1Centre régional de transfusion sanguine, Madinat Al Irfane, BP 180, 10000 Rabat, Maroc. s.achargui@menara.ma
Aim Of The Study:
Free fetal DNA in maternal blood offers a non invasive method for prenatal diagnosis. The aim of this study is to perform fetal RHD genotyping by conventional PCR in D negative pregnant women of Moroccan origin.
Patients And Methods:
Plasma sample from 120 D negative pregnant women from 10 to 40 gestational weeks were tested by conventional PCR for the presence of exons 7 and 10 of RHD gene. The results were compared with the RhD phenotype of the newborns.
Results:
In this study, the positive and the negative predictive value of the fetal RhD status were 98.7 and 96.7%, respectively.
Conclusion:
These results demonstrate de feasibility of fetal RHD genotyping by conventional PCR in D negative pregnant women of Moroccan origin.
