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Updated: Jun 5, 2026

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

Published on: January 29, 2019

Tomosyn expression pattern in the mouse hippocampus suggests both presynaptic and postsynaptic functions.

Boaz Barak¹, Antionette Williams, Noa Bielopolski

¹Department of Neurobiology, The George S. Wise Faculty of Life Sciences, Tel-Aviv University Tel Aviv, Israel.

Frontiers in Neuroanatomy

|December 31, 2010

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Tomosyn protein regulates neuronal communication. This study reveals tomosyn1 is mainly at presynaptic sites, while tomosyn2 may also function postsynaptically, suggesting a broader role in synaptic plasticity.

Area of Science:

Neuroscience
Molecular Biology
Synaptic Function

Background:

Tomosyn protein is known to modulate synaptic transmission and vesicle release probability.
Its precise localization and function within specific neuronal compartments require further elucidation.

Purpose of the Study:

To detail the expression and localization patterns of tomosyn1 and tomosyn2 in distinct mouse hippocampal subareas.
To investigate the potential presynaptic and postsynaptic roles of tomosyn isoforms.

Main Methods:

Confocal and two-photon high-resolution microscopy.
Immunohistochemical analysis using pre- and postsynaptic markers.
Examination of tomosyn localization in hippocampal subregions.

Main Results:

Keywords:

CA2 area hippocampus mossy fiber terminals synaptic protein tomosyn isoforms

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Published on: March 13, 2016

Related Experiment Videos

Last Updated: Jun 5, 2026

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

Published on: January 29, 2019

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

Published on: October 6, 2017

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

Published on: March 13, 2016

Tomosyn isoforms (tomosyn1 and tomosyn2) were localized within glutamatergic synapses in the mouse hippocampus.
Tomosyn1 showed differential distribution, concentrated in the hilus and mossy fibers.
Tomosyn2 was found in pyramidal cell bodies, dendrites, and spines, colocalizing with PSD95, indicating a potential postsynaptic role.

Conclusions:

Tomosyn1 primarily functions presynaptically, regulating neurotransmitter release.
Tomosyn2 exhibits a potential postsynaptic role in addition to presynaptic functions.
Tomosyn acts as a versatile regulator of synaptic transmission and plasticity.