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Updated: Jun 5, 2026
A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
Published on: December 7, 2016
Analytical evaluation of the Tosoh HLC-723 G8 automated HPLC analyzer for hemoglobin analysis in beta-thalassemia
Françoise Merono1, Imane Agouti, Nathalie Bonello-Palot
1UMR 910, Faculté de Médecine, Université de la Méditerranée, Marseille, France.
Objectives:
The analytical performances of a new kit conceived for Hb variants separation and measurement procedures on an HPLC instrument (Tosoh HLC-723 G8) were studied.
Results:
Between-run and within-run precision tests were satisfactory for both HbA2 and HbF measurements. HbA2 and HbF values measured using the TOSOH HLC-723 G8 were correlated to those obtained using the Bio-Rad Variant II HPLC system (r=0.974 and 0.997 respectively) and to those given by the Sebia Capillary's system (r=0.980 and 0.996 respectively). Linearity was observed for HbA2 from 2.24% to 6.56% and for HbF from 0.5 to 6%.
Conclusion:
The new analyzer Tosoh HLC-723 G8 was found to have a wide analytical range for both HbA2 and HbF. The G8 Mode beta-thal is suitable for a first-level laboratory screening for Hb analysis but also for a second-level test for the characterization of Hb variants after a first-line screening.
