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The lysosomal polypeptide transporter TAPL: more than a housekeeping factor?
Irina Bangert1, Franz Tumulka, Rupert Abele
1Institute of Biochemistry, Biocenter, Goethe University Frankfurt, Max-von-Laue-Strasse 9, Frankfurt/Main, Germany.
Biological Chemistry
|January 4, 2011
Summary
The transporter associated with antigen processing-like (TAPL) is an ATP-driven polypeptide transporter found across diverse species. This study details TAPL
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- The transporter associated with antigen processing-like (TAPL) is an ATP-binding cassette (ABC) transporter.
- TAPL functions as an intracellular polypeptide transporter, moving peptides into lysosomes.
- It forms a homodimer and exhibits broad tissue and phylogenetic distribution.
Purpose of the Study:
- To elucidate the topology, substrate specificity, and distribution of the TAPL transporter.
- To discuss the proposed physiological roles of TAPL.
- To explore TAPL's involvement in housekeeping, metabolite storage, and adaptive immunity.
Main Methods:
- Biochemical assays to determine transporter function.
- Structural analysis to define topology.
- Phylogenetic analysis to assess distribution.
- Functional studies to investigate substrate specificity.
Main Results:
- Detailed characterization of TAPL's membrane topology.
- Identification of specific peptide substrates translocated by TAPL.
- Confirmation of TAPL's presence in various tissues and across different species, including nematodes and insects.
- Evidence supporting TAPL's roles in cellular housekeeping and immune functions.
Conclusions:
- TAPL is a crucial intracellular transporter with significant roles in cellular processes.
- Its broad distribution suggests fundamental importance in eukaryotic biology.
- Further research into TAPL's function can provide insights into lysosomal transport and immunity.
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