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Updated: Jun 5, 2026
Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice
Published on: March 11, 2020
Simultaneous monitoring of excitatory postsynaptic potentials and extracellular L-glutamate in mouse hippocampal
Shizuko Hozumi1, Kana Ikezawa, Atushi Shoji
1Department of Chemistry, College of Humanities and Sciences, Nihon University, Sakurajuosui, Setagayaku, Tokyo 156-8550, Japan.
Abstract:
Simultaneous monitoring of amperometric currents at a glass capillary sensor based on recombinant GluOx and field excitatory postsynaptic potentials (fEPSPs) were performed in region CA1 of mouse hippocampal slices. A transient increase in the glutamate current relative to the basal one at control stimulation (0.052Hz) was evoked by stimulation at 2 Hz for 2 min. The magnitude of the glutamate current was dependent on the intensity (current) of a 2 Hz stimulus and reflected the slope of the fEPSP. The in situ calibration of the L-glutamate sensor revealed that the extracellular concentration of L-glutamate released by 2 Hz stimulation before tetanus is in the range from 0.8 to 2.2 microM and it is enhanced after tetanic stimulation. The L-glutamate level at a test stimulus (0.052 Hz) was estimated to be 32 nM. The recombinant GluOx-based sensor exhibited weak responses to glutamine above 300 microM and L-aspartic acid above 200 microM. The potential use of a glass capillary sensor in combination with fEPSP measurements for electrophysiological study is discussed.
