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Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
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Recombinant DNA01:09

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Related Experiment Video

Updated: Jun 5, 2026

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

Serine recombinases as tools for genome engineering.

William R A Brown1, Nicholas C O Lee, Zhengyao Xu

  • 1School of Biology, Queens Medical Centre, Nottingham NG7 2UH, UK. William.brown@nottingham.ac.uk

Methods (San Diego, Calif.)
|January 4, 2011
PubMed
Summary

Serine recombinases, like ϕC31 integrase, perform unidirectional reactions, differing from tyrosine recombinases. Their unique mechanisms offer opportunities for engineering novel enzymes for genome engineering applications.

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Principles of Site-Specific Recombinase (SSR) Technology
07:06

Principles of Site-Specific Recombinase (SSR) Technology

Published on: May 29, 2008

Related Experiment Videos

Last Updated: Jun 5, 2026

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

Principles of Site-Specific Recombinase (SSR) Technology
07:06

Principles of Site-Specific Recombinase (SSR) Technology

Published on: May 29, 2008

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Serine recombinases, including ϕC31 integrase, represent a distinct class of enzymes compared to tyrosine recombinases.
  • Unlike Cre and Flp recombinases, serine recombinases catalyze unidirectional reactions.
  • This family encompasses numerous proteins with significant potential for genome engineering.

Purpose of the Study:

  • To review the reaction mechanisms of serine recombinases.
  • To explore how these mechanisms both limit and enable enzyme engineering.
  • To discuss current challenges and future prospects in serine recombinase applications for genome engineering.

Main Methods:

  • Review of existing literature on serine recombinase mechanisms.
  • Analysis of reaction characteristics and engineering potential.
  • Discussion of experimental findings and newly discovered enzymes.

Main Results:

  • Serine recombinase mechanisms, while limiting in some aspects, present opportunities for enzyme design.
  • Unanswered questions persist regarding their application in diverse genome engineering systems.
  • Recently identified serine recombinases show promise for future genome engineering endeavors.

Conclusions:

  • Understanding serine recombinase mechanisms is crucial for advancing genome engineering.
  • The unique properties of serine recombinases can be harnessed for novel enzyme development.
  • Continued exploration of this enzyme family will expand their utility in biotechnology.