Estimation of paraproteins by immunoturbidimetry and electrophoresis followed by scanning densitometry

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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
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D Sinclair1, F Ballantyne, S Shanley

  • 1Institute of Biochemistry, Royal Infirmary, Glasgow, UK.

Annals of Clinical Biochemistry
|July 1, 1990
PubMed

A comparison of paraprotein estimation by immunoturbidimetry (IT) and electrophoresis followed by scanning densitometry (EFD) showed significant differences in concentration for IgG, IgA, and IgM paraproteins. Overestimation of IgM paraproteins by IT was particularly marked. Our data suggest that both techniques should be used for IgG and IgA paraproteins but for IgM paraproteins IT was found to be unsuitable and EFD is the method of choice.

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