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Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
In vitro Mutagenesis01:16

In vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Crossing Over01:30

Crossing Over

Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I, duplicated...

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Related Experiment Video

Updated: Jun 5, 2026

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
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Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction

Published on: November 20, 2018

Cre recombinase resources for conditional mouse mutagenesis.

Damian Smedley1, Ekaterina Salimova, Nadia Rosenthal

  • 1European Bioinformatics Institute, Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK.

Methods (San Diego, Calif.)
|January 4, 2011
PubMed
Summary
This summary is machine-generated.

Conditional mutagenesis in mice is essential for modeling human diseases. This review details Cre driver mouse lines and databases, crucial for advancing genetic research and drug discovery.

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Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation
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Last Updated: Jun 5, 2026

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
08:01

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Published on: November 20, 2018

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
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Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

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Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation
10:29

Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation

Published on: November 17, 2017

Area of Science:

  • Genetics and Genomics
  • Developmental Biology
  • Pharmacology

Background:

  • The mouse is a key model organism for human disease and drug action studies.
  • Conditional mutagenesis enables precise gene function studies, especially for genes with pleiotropic effects.
  • Cre/loxP technology is central to creating conditional mouse mutants with spatial and temporal control.

Purpose of the Study:

  • To provide an updated overview of global Cre driver mouse lines.
  • To review public databases and portals for Cre driver line information.
  • To highlight the importance of well-characterized Cre driver lines for conditional mutagenesis.

Main Methods:

  • Systematic review of international activities in conditional mouse mutagenesis.
  • Analysis of Cre driver mouse line characteristics (efficiency, specificity, genetic background).
  • Evaluation of public databases and portals for Cre driver line data.

Main Results:

  • Significant advancements in sophisticated mouse genome manipulation.
  • The majority of new mouse mutants are conditional, utilizing Cre/loxP systems.
  • Availability of well-characterized Cre driver lines is critical for exploiting conditional mutant mice.

Conclusions:

  • Cre driver mouse lines are indispensable tools for conditional and inducible mutagenesis.
  • Public databases and portals are vital resources for researchers utilizing Cre driver lines.
  • The rapid progression of research necessitates continuous updates on available mouse resources.