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Bromothymol blue and carbohydrate-sensitive plating media.

M R DuPree, G Wilcox

    Journal of Clinical Microbiology
    |October 1, 1977
    PubMed
    Summary
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    A novel bromothymol blue (BTB) plating medium enhances bacterial differentiation. This BTB medium detects carbohydrate fermentation at significantly lower concentrations than conventional enteric media, improving microbial analysis.

    Area of Science:

    • Microbiology
    • Biochemistry

    Background:

    • Conventional enteric media like EMB, MacConkey, and Endo are widely used for bacterial differentiation.
    • Optimizing media for sensitive detection of carbohydrate fermentation is crucial in microbiology.

    Purpose of the Study:

    • To introduce and evaluate a new plating medium utilizing bromothymol blue (BTB) indicator.
    • To compare the efficacy of BTB medium against established enteric media for bacterial strain differentiation.
    • To determine the minimum carbohydrate concentrations required for effective differentiation using BTB and modified conventional media.

    Main Methods:

    • Comparison of BTB medium with eosin-methylene blue (EMB), MacConkey, and Endo media.
    • Testing with L-arabinose using fermenting and nonfermenting mutant strains of Escherichia coli.

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  • Determination of minimum effective carbohydrate concentrations for differentiating various colony types and testing L-arabinose, L-rhamnose, D-lactose, and D-galactose.

    • Main Results:

    • The new BTB medium demonstrated effective differentiation of Escherichia coli strains based on L-arabinose fermentation.
    • Minimum concentrations for differentiation varied depending on colony spacing (confluent, isolated, or adjacent).
    • BTB and reformulated conventional media detected acidification at 1/5th to 1/100th the carbohydrate concentration of standard indicator plates.

    Conclusions:

    • Bromothymol blue (BTB) indicator offers a sensitive and effective alternative for bacterial plating media.
    • The BTB medium allows for microbial differentiation at significantly reduced carbohydrate concentrations, enhancing sensitivity and potentially reducing costs.
    • This advancement in plating media can improve the detection and analysis of bacterial fermentation characteristics.