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Neuronal expression of inducible nitric oxide synthase in hypothyroid rat
1Department of Medicine, The Children's Hospital of Zhejiang University School of Medicine, China.
Objectives:
The expression of iNOS mRNA and protein levels was studied in hypothyroid rat cerebral cortex during early days after postnatal life.
Method:
The homogenate of the cerebral cortex was examined using Real-time reverse transcription polymerase chain reaction (real-time RT PCR) and Western blot analysis.
Result:
ΔCt in hypothyroid group on day 7, 14, 21 was 18.89±0.92, 18.83±0.99, 16.48±0.29, separately. While in control group, ΔCt was 20.32±0.92, 20.07±0.86, 17.96±0.50, separately. Meanwhile, the protein level was detected only in hypothyroid group of 59 ± 5% on day 14. On day 21, iNOS protein expression showed a decrease (p<0.05) in hypothyroid group (37 ± 3%), compared with control group (58 ± 4%).
Conclusion:
Our findings show the successive changes of the iNOS mRNA and protein levels in early postnatal days of hypothyroid rat brain, and confirm the cross-talk between the TH and NO signaling pathway in developing cerebral cortex of rats.
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