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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 5, 2026

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

Two-photon spectral imaging with high temporal and spectral resolution.

Kang-Bin Im1, Moon-Sik Kang, Jiho Kim

  • 1Neurodegeneration and Applied Microscopy, Institut Pasteur Korea, Seongnam, Gyeonggi-do, Korea.

Optics Express
|January 4, 2011
PubMed
Summary

We developed a rapid spectral imaging system using an electron-multiplying charge-coupled device (EM-CCD) detector. This advanced technology enables high-resolution imaging of biological samples, including living cells and tissues.

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In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy
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In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy

Published on: January 19, 2011

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Last Updated: Jun 5, 2026

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy
14:26

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy

Published on: January 19, 2011

Area of Science:

  • Biophotonics
  • Microscopy
  • Spectroscopy

Background:

  • Advanced imaging techniques are crucial for understanding cellular and tissue dynamics.
  • Existing spectral imaging systems often face limitations in speed or spectral resolution.
  • The need for faster, high-resolution methods to study biological processes in real-time is growing.

Purpose of the Study:

  • To introduce a novel, fast spectral imaging system.
  • To achieve high spectral resolution and rapid frame acquisition simultaneously.
  • To demonstrate the system's utility in live biological sample analysis.

Main Methods:

  • Integration of an electron-multiplying charge-coupled device (EM-CCD) detector.
  • Combination with a custom-built two-photon excitation laser scanning microscope.
  • Implementation of 80 detection channels for comprehensive spectral data capture.

Main Results:

  • The system achieves fast frame acquisition without compromising spectral information.
  • Successful monitoring of fluorescent proteins and quantum dot-labeled G protein-coupled receptors in living cells.
  • Effective detection of autofluorescence in tissue samples.

Conclusions:

  • The developed spectral imaging system offers a powerful tool for biological research.
  • Its speed and spectral resolution enable new possibilities in live-cell and tissue imaging.
  • This technology advances the study of dynamic biological processes.