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Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Viral Recombination00:57

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.

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Related Experiment Video

Updated: Jun 5, 2026

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
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Published on: January 8, 2015

Bacterial artificial chromosome mutagenesis using recombineering.

Kumaran Narayanan1, Qingwen Chen

  • 1Department of Genetics and Genomic Sciences, Mount Sinai School of Medicine, New York, NY 10029, USA. kumaran.narayanan@sci.monash.edu.my

Journal of Biomedicine & Biotechnology
|January 4, 2011
PubMed
Summary

Bacterial artificial chromosomes (BACs) enable accurate gene expression. Recombineering techniques now allow BAC modification, expanding functional genetics research possibilities.

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Last Updated: Jun 5, 2026

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

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Published on: January 8, 2015

Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone
08:10

Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone

Published on: June 24, 2019

Area of Science:

  • Molecular Biology
  • Genetics
  • Genomics

Background:

  • Bacterial artificial chromosomes (BACs) facilitate physiologically relevant gene expression.
  • BACs transfer intact genes with regulatory elements for precise spatiotemporal control.
  • Conventional genetic engineering methods are limited for modifying large BAC DNA.

Purpose of the Study:

  • To overcome limitations in modifying BAC DNA for functional studies.
  • To enable facile engineering of high molecular weight BAC DNA.
  • To expand the utility of BACs in functional genetics research.

Main Methods:

  • Development of in vivo homologous recombination strategies.
  • Application of recombineering techniques in E. coli.
  • Engineering of BAC DNA without reliance on restriction enzymes or cloning.

Main Results:

  • Successful modification of large BAC DNA constructs.
  • Facilitation of BAC engineering independent of restriction sites.
  • Expanded capabilities for in vitro and in vivo functional genetics studies.

Conclusions:

  • Recombineering has resolved major obstacles in BAC DNA engineering.
  • BACs are now more accessible for advanced functional genetics research.
  • These advancements significantly broaden the application scope of BAC technology.