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Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Related Experiment Video

Updated: Jun 5, 2026

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
10:06

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

Ferrule-top atomic force microscope.

D Chavan1, G Gruca, S de Man

  • 1Faculty of Sciences, Department of Physics and Astronomy and LaserLaB, Vrije Universiteit, Amsterdam, The Netherlands.

The Review of Scientific Instruments
|January 5, 2011
PubMed
Summary
This summary is machine-generated.

Ferrule-top cantilevers enable ultra-compact atomic force microscopy (AFM) for imaging in air and liquids. These all-optical devices offer sensitivity comparable to commercial AFMs and are easy to handle.

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Area of Science:

  • Microscopy and Nanotechnology
  • Optical Physics
  • Materials Science

Background:

  • Ferrule-top cantilevers are all-optical miniaturized devices designed for use in liquids and harsh environments.
  • They are fabricated by shaping the end of a ferruled fiber into a mechanical beam, enabling optical detection of deflections.

Purpose of the Study:

  • To demonstrate the utility of ferrule-top cantilevers for developing ultra-compact atomic force microscopes (AFMs).
  • To evaluate their performance in contact mode imaging in both air and liquid environments.

Main Methods:

  • Fabrication of ferrule-top cantilevers by carving ferruled fiber ends into mechanical beams.
  • Utilizing light coupled through the fiber for detecting cantilever deflections.
  • Performing contact mode imaging in air and liquids using the developed AFM system.

Main Results:

  • Ferrule-top cantilevers facilitate the development of ultra-compact AFMs.
  • The developed AFM system achieves sensitivity comparable to commercial AFMs.
  • The probes demonstrate ease of handling and require no alignment procedures.

Conclusions:

  • Ferrule-top cantilevers are suitable for creating highly sensitive, compact AFM systems.
  • Their robustness and ease of use expand AFM applications beyond traditional laboratory settings.
  • These devices offer a promising platform for advanced imaging in diverse environments.