Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SPOTTER: Automated Tissue-Barcoding Platform for Spatial Proteomics and Phosphoproteomics.

bioRxiv : the preprint server for biology·2026
Same author

Beyond Accuracy: Safety-Centered guidelines for the evaluation of LLM-based therapy recommendation systems for chronic multimorbidity patients.

Journal of biomedical informatics·2026
Same author

ADGRF4 and ADGRL4 as novel prognostic biomarkers and potential therapeutic implications in stomach adenocarcinoma.

BMC gastroenterology·2026
Same author

A 3D-printed osteochondral scaffold with a dual biomimetic design of spatially organized lotus-radial microchannels and bioinspired nano-mineral precursors for efficient osteochondral regeneration.

Biofabrication·2026
Same author

Efficient spatio-angular reconstruction enables high-fidelity mapping of six-dimensional structures and dynamics with polarized fluorescence microscopy.

Research square·2026
Same author

Super-Resolution Structured Illumination Microscopy to Study Endocytosis and Cellular Nanoparticle Uptake.

ACS nano·2026

Related Experiment Video

Updated: Jun 5, 2026

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
10:35

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

Fiber-optic nonlinear endomicroscopy with focus scanning by using shape memory alloy actuation.

Yicong Wu1, Yuying Zhang, Jiefeng Xi

  • 1Johns Hopkins University, Department of Biomedical Engineering, Baltimore, MD 21205, USA.

Journal of Biomedical Optics
|January 5, 2011
PubMed
Summary

A novel miniature fiber optic endomicroscope enables 3-D two-photon fluorescence imaging. This system utilizes shape memory alloy for dynamic axial scanning, advancing biological sample visualization.

More Related Videos

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
13:49

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

Video-rate Scanning Confocal Microscopy and Microendoscopy
14:10

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

Related Experiment Videos

Last Updated: Jun 5, 2026

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
10:35

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
13:49

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

Video-rate Scanning Confocal Microscopy and Microendoscopy
14:10

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

Area of Science:

  • Biomedical Engineering
  • Optical Imaging
  • Materials Science

Background:

  • Advanced imaging techniques are crucial for visualizing biological structures at high resolution.
  • Current fiber optic endomicroscopes face limitations in achieving dynamic 3-D scanning capabilities.
  • Two-photon fluorescence (TPF) microscopy offers advantages for deep tissue imaging due to reduced scattering and photobleaching.

Purpose of the Study:

  • To develop a miniature fiber optic endomicroscope with integrated dynamic focus scanning for 3-D TPF imaging.
  • To demonstrate the feasibility of using shape memory alloy (SMA) for precise axial scanning in an endomicroscopic probe.
  • To achieve high-resolution, depth-resolved 3-D imaging of biological samples.

Main Methods:

  • Development of a miniature fiber optic endomicroscope incorporating a double-clad fiber cantilever for lateral beam scanning via a piezoactuator.
  • Integration of a compact, electrically driven shape memory alloy (SMA) actuator for controlled axial scanning of the imaging probe.
  • Calibration of the nonlinear but repeatable SMA contraction response to applied voltage for precise focus control.
  • Performance evaluation using depth-resolved 3-D TPF imaging of acriflavine-stained biological tissues and unstained white paper.

Main Results:

  • Successful development of a miniature fiber optic endomicroscope with built-in dynamic focus scanning.
  • Demonstration of fast 2-D lateral scanning using a vibrating fiber cantilever and piezoactuator.
  • Achieved slow axial scanning with a 150-μm contraction range using a compact SMA actuator (50-100 mV).
  • Obtained depth-resolved 3-D TPF images of biological tissues and paper, confirming system feasibility.

Conclusions:

  • The developed SMA-based scanning fiber-optic endomicroscope is a feasible platform for 3-D nonlinear optical imaging.
  • The integrated dynamic focus scanning capability enhances the 3-D imaging potential of fiber optic endomicroscopy.
  • This technology holds promise for in vivo biological imaging and diagnostics requiring high-resolution 3-D visualization.