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Updated: Jun 5, 2026

Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb
Published on: December 2, 2017
Simultaneous assessment of pulsating and total blood in inflammatory skin lesions using functional diffuse
InSeok Seo1, Paulo R Bargo, Nikiforos Kollias
1Johnson and Johnson Consumer Products Company, Skillman, NJ 08558, USA. iseo@its.jnj.com
Abstract:
We present a simple and cost-effective optical technique for the simultaneous assessment of pulsating and total blood noninvasively in an inflammatory skin lesion. Acquisitions of diffuse reflectance spectra in the visible range at 6 Hz are used to trace the oscillating components of reflectance. Measurements on erythematous lesions from a UV insult show slow changing signal at about 0.1 Hz and heart-driven regular oscillations at about 1 Hz simultaneously. The results demonstrate the potential of the technique in monitoring both pulsating and steady components of the blood in inflammatory lesions of the skin.
