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Updated: Jan 17, 2026

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
Published on: September 13, 2022
Localization capability and limitation of electron-multiplying charge-coupled, scientific complementary metal-oxide
Tingwei Quan1, Shaoqun Zeng, Zhen-Li Huang
1Huazhong University of Science and Technology,, Wuhan National Laboratory for Optoelectronics, Wuhan 430074, China.
Abstract:
Localization of a single fluorescent molecule is required in a number of superresolution imaging techniques for visualizing biological structures at cellular and subcellular levels. The localization capability and limitation of low-light detectors are critical for such a purpose. We present an updated evaluation on the performance of three typical low-light detectors, including a popular electron-multiplying CCD (EMCCD), a newly developed scientific CMOS (sCMOS), and a representative cooled CCD, for superresolution imaging. We find that under some experimental accessible conditions, the sCMOS camera shows a competitive and even better performance than the EMCCD camera, which has long been considered the detector of choice in the field of superresolution imaging.
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