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Related Concept Videos

Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...

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Related Experiment Video

Updated: Jun 5, 2026

Optimized PCR-based Detection of Mycoplasma
06:01

Optimized PCR-based Detection of Mycoplasma

Published on: June 20, 2011

Decontamination efficacy against Mycoplasma.

M Eterpi1, G McDonnell, V Thomas

  • 1STERIS SA R&D, Fontenay-aux-Roses, France.

Letters in Applied Microbiology
|January 5, 2011
PubMed
Summary

Ethanol, alkaline cleaners, and vaporized hydrogen peroxide effectively eliminate mycoplasma contamination. Quaternary ammonium compounds showed poor efficacy against these resilient bacteria, highlighting the need for optimized disinfection strategies.

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Last Updated: Jun 5, 2026

Optimized PCR-based Detection of Mycoplasma
06:01

Optimized PCR-based Detection of Mycoplasma

Published on: June 20, 2011

Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae
06:04

Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae

Published on: February 24, 2023

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Biotechnology

Background:

  • Mycoplasma are ubiquitous microorganisms found in various environments and tissues.
  • They pose public health risks, are linked to cancers, and are a significant contamination concern in biotechnology.
  • Mycoplasma's small size, biofilm formation, and survival on dried surfaces present disinfection challenges.

Purpose of the Study:

  • To evaluate the efficacy of commonly used biocidal treatments against three representative mycoplasma species.
  • To assess the resistance of mycoplasma to environmental factors and disinfection methods.

Main Methods:

  • Mycoplasma strains were dried onto stainless steel coupons.
  • Coupons were exposed to various decontamination products, including quaternary ammonium compounds (QAC), 70% ethanol, alkaline cleaners, and vaporized hydrogen peroxide (VHP).
  • Viability loss was assessed to determine disinfectant efficacy.

Main Results:

  • All tested mycoplasma strains survived drying with minimal viability loss.
  • 70% ethanol demonstrated complete inactivation within 5 minutes.
  • Alkaline cleaners showed increasing efficacy with concentration, achieving complete kill at 0.8% for two products.
  • Vaporized hydrogen peroxide was highly effective for room disinfection and device sterilization.

Conclusions:

  • Ethanol and alkaline detergent formulations are efficient disinfectants against mycoplasma.
  • Vaporized hydrogen peroxide is effective for both room disinfection and device sterilization.
  • Quaternary ammonium compounds were found to be ineffective, indicating mycoplasma's resistance to certain biocides.