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Related Concept Videos

Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Cohesins02:20

Cohesins

Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...

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Related Experiment Video

Updated: Jun 5, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Anaphase-promoting complex/cyclosome controls HEC1 stability.

L Li1, Y Zhou, G-F Wang

  • 1Laboratory of Cell Senescence, Shantou University Medical College, Shantou, Guangdong, China.

Cell Proliferation
|January 5, 2011
PubMed
Summary
This summary is machine-generated.

The anaphase-promoting complex/cyclosome (APC/C)-Cdh1 E3 ligase targets the kinetochore protein HEC1 for degradation, ensuring proper cell division. Disrupting this process causes mitotic arrest.

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Last Updated: Jun 5, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
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Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Kinetochore proteins are crucial for accurate chromosome segregation during mitosis.
  • HEC1 (high expression in cancer) is a vital kinetochore component.
  • Dysregulation of kinetochore proteins leads to cell cycle arrest and death.

Purpose of the Study:

  • To investigate the post-translational regulation of HEC1 protein.
  • To determine the role of the anaphase-promoting complex/cyclosome (APC/C)-Cdh1 in HEC1 degradation.

Main Methods:

  • RNA interference (RNAi) was used to assess HEC1 stability.
  • In vivo ubiquitination assays were performed.
  • HEK293T cells were utilized to study endogenous HEC1 regulation.

Main Results:

  • HEC1 is an unstable protein targeted by the ubiquitin-proteasome system.
  • APC/C-Cdh1 E3 ligase ubiquitinates and degrades HEC1.
  • A conserved D-box at HEC1's C-terminus acts as a degron, mediating APC/C-Cdh1 degradation; its destruction confers resistance.
  • Overexpression of a non-degradable HEC1 mutant caused cyclin B accumulation and mitotic arrest.

Conclusions:

  • APC/C-Cdh1 controls HEC1 protein stability.
  • This regulation is essential for normal cell cycle progression.
  • Targeted degradation of HEC1 by APC/C-Cdh1 prevents mitotic errors.