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Updated: Jun 5, 2026

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
Published on: March 19, 2018
Evaluation of a shortened QIAsymphony DNA extraction protocol for stool samples using a multiplex real-time PCR for
E van Zanten1, G J Wisselink, S Stoll
1Laboratory for Infectious Diseases, Department of Research and Development, Groningen, The Netherlands. e.van.zanten@infectielab.nl
Abstract:
A shortened DNA extraction protocol for the QIAsymphony SP was evaluated by quantitative and qualitative comparison of real-time PCR results of 150 co-extracted stool samples. The average ∆Cycle threshold value for positive pathogenic targets was 0.28 Ct. A consensus of 96.91%, with a correlation coefficient of 0.9880 was recorded.
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