[Cytocompatibility of dense polycrystalline hydroxyapatite particles as bone substitute]

The vitro cell culture was used to study cytocompatibility of dense polycrystalline hydroxylapatite. Histologic examination and scanning electron microscopy were used to study qualitatively the growth and adhesion of fibroblasts when they came into contact with hydroxylapatite in culture. The proliferation of fibroblasts in vitro, using the tritiated thymidine uptake method, allows a quantitative analysis of cell activation in response to the extracted solution of the materials. The results of experiment indicated that there were no adverse effects on the growth, adhesion and proliferation rate of human gingiva fibroblasts when they came into contact with hydroxylapatite.