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Updated: Jun 6, 2026

Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes
Published on: February 22, 2017
In vivo activation by ultraviolet rays of the human immunodeficiency virus type 1 long terminal repeat
1Unité 152 Institut Cochin de Génétique Moléculaire, Institut National de la Santé et de la Recherche Medical, Paris, France.
Abstract:
It has been previously shown in vitro that the human immunodeficiency virus type 1 long terminal repeat (LTR) is activated by ultraviolet irradiation. In order to analyze if a similar effect could occur in vivo, transgenic mice carrying the lacZ gene under the control of the viral LTR were irradiated at 280-300 and 254 nm. These mice spontaneously expressed the transgene in the epidermis and the lens of both adults and embryos. Irradiations caused a significant increase in skin beta-galactosidase activity. This phenomenon might be involved in viral activation and could be of interest in regard to the skin pathology observed during an HIV infection.
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