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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 5, 2026

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

Methods for microRNA microarray profiling.

Aarati R Ranade¹, Glen J Weiss

¹Translational Genomics Research Institute, Phoenix, AZ, USA.

Methods in Molecular Biology (Clifton, N.J.)

|January 5, 2011

Summary

This summary is machine-generated.

MicroRNAs (miRNAs) are key regulators of gene expression. This study details a microarray method for profiling miRNAs, aiding in cancer biomarker discovery and therapeutic development.

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Last Updated: Jun 5, 2026

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

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Published on: February 21, 2014

Performing Custom MicroRNA Microarray Experiments

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Published on: October 28, 2011

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Published on: December 3, 2010

Area of Science:

Molecular Biology
Genomics
Biochemistry

Background:

MicroRNAs (miRNAs) are small, single-stranded RNA molecules regulating gene expression.
Emerging research highlights miRNA involvement in oncogenesis and potential as cancer biomarkers.
Microarray analysis is a powerful tool for identifying candidate miRNAs.

Purpose of the Study:

To present a detailed protocol for miRNA microarray profiling.
To facilitate the identification of disease-specific miRNA signatures.
To support the development of miRNA-based cancer diagnostics and therapeutics.

Main Methods:

Utilizing the Agilent microarray platform for miRNA expression profiling.
Implementing a standardized protocol for robust data generation.
Correlating miRNA expression patterns with biological processes like carcinogenesis.

Main Results:

The presented protocol enables comprehensive miRNA profiling.
Identification of candidate miRNAs involved in cancer signaling pathways.
Establishment of a foundation for developing molecular signatures for disease states.

Conclusions:

Microarray-based miRNA profiling is a valuable strategy for cancer research.
The Agilent platform offers a reliable method for miRNA analysis.
This protocol can advance the discovery of miRNA biomarkers for cancer diagnosis, prognosis, and therapy.