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Specific mRNAs accumulate in long-wavelength UV-irradiated mouse epidermis
1Department of Biology, Laboratoire de Recherche Fondamentale de l'Oréal, Aulnay-sous-Bois, France.
Abstract:
Total RNA from hairless mice epidermis was analysed by RNA-DNA hybridization on slot blots at various times following irradiation with long-wavelength UV light (UV-B or UV-B plus UV-A). Densitometric scanning of the autoradiographies indicates that the amount of RNA which hybridizes with an Ha-ras DNA probe remains constant for up to 72 h after irradiation. The amount of the transcript which hybridizes to a c-fos DNA probe increases dose dependently from 15 min to 2 h after irradiation. RNA which hybridizes to a tubulin DNA probe accumulates in parallel with the increase in mitotic index associated with the radiation-induced hyperplasia.