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09:39
A Platform of Anti-biofilm Assays Suited to the Exploration of Natural Compound Libraries
Published on: December 27, 2016
Incorporation of secretory immunoglobulin A into biofilms can decrease their resistance to ciprofloxacin.
Yu-Huei Lee1, Kuei-Ying Su, Aaron Wyse
1Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA.
Microbiology and Immunology
|January 6, 2011
Summary
Symbiotic biofilms incorporating host molecules like secretory IgA (SIgA) may show reduced antibiotic resistance. This contrasts with planktonic bacteria, where SIgA can increase resistance, impacting medical and biological understanding.
Area of Science:
- Microbiology
- Biochemistry
- Medical Science
Background:
- Bacterial biofilms on inert surfaces produce their own extracellular matrices.
- Symbiotic biofilms utilize host-derived macromolecules in their extracellular matrices.
- Host molecules can significantly influence symbiotic biofilm antibiotic resistance.
Purpose of the Study:
- To investigate the effect of host-derived molecules on biofilm antibiotic sensitivity.
- To evaluate ciprofloxacin's efficacy against biofilms with and without secretory IgA (SIgA).
Main Methods:
- An in vitro model was employed.
- Biofilms of Escherichia coli were grown with and without SIgA.
- Antibiotic susceptibility to ciprofloxacin was assessed.
Main Results:
- Five of six E. coli strains showed decreased ciprofloxacin resistance when SIgA was incorporated into biofilms.
- SIgA generally increased planktonic bacteria's resistance to ciprofloxacin.
- SIgA-mediated bacterial aggregation may contribute to increased planktonic resistance.
Conclusions:
- Host-derived molecules in symbiotic biofilm matrices can alter biofilm properties.
- Incorporation of SIgA into extracellular matrices may reduce biofilm resistance to antibiotics.
- Findings have implications for understanding symbiotic biofilms in medicine and biology.
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