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Published on: September 25, 2019
[Effect of hepatitis B virus X gene on the expression of spastic paraplegia 21]
Guo-sheng Gao1, Peng-jian Weng, Reng-bin Ji
1The Infectious Disease Hospital of Ningbo, Ningbo 315010, China.
Objective:
To investigate the effect of hepatitis B virus(HBV) X gene on the expression of SPG21.
Methods:
The expressions of SPG21 mRNA and protein in HepG2 and HepG2.2.15 cells were tested by RT-PCR and western blot. HepG2 cells were co-transfected with reporter plasmid pGL3-SPG21 and plasmids carrying individual genes of HBV, the luciferase activity was measured and the expressions of SPG21 were detected by RT-PCR and western blot.
Results:
The expressions of SPG21 mRNA and protein were higher in HepG2.2.15 cells than in HepG2 cells (0.36+/-0.06 vs 0.21+/-0.05, P value is less than 0.05). The activity of SPG21 in HepG2 cells transfected with pCMV-X was higher (875+/-27 vs 67+/-12, P value is less than 0.01) as compared to blank control group (transfected with pCMV-tag2B). HBV X gene enhanced SPG21 gene promoter activity, SPG21 mRNA expression and SPG21 protein production in HepG2 cells in a dose-dependent manner.
Conclusion:
HBV X gene can specially activate SPG21 expression.
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